Pharmacokinetic studies of “off-label” drugs in food producing animals: quantification of drug residues in different organic matrices

Four studies were carried out in the present thesis: In the first study 65 poultry chickens were randomly divided into 4 groups: group A (n = 20) and B (n = 20) received the drug orally (PO), in group C (n = 20) the drug was injected by intravenous (IV) route, whereas group D (n = 5) was the control group. The analysis of plasma samples and residues in organs were performed through the HPLC-FL instrument. The second study was divided into 2 phases where at the same 6 lactating goats were given tulathromycin drug before gestation and during lactation postpartum period. In the first phase of the study, tulathromycin was administered at 2.5 mg/kg b.w. by IV and subcutaneous (SC) routes. In the second phase of the study, the same animals were administered with a single IV dose. The analysis of plasma samples and organs were performed through the HPLC-MS/MS instrument. In the third study, 20 laying hens were divided into 3 groups: group A (n=6), B (n=6) and C (n=8). During the first phase group A and B was administered the drug tapentadol by IV and PO routes at the dose of 1 and 5 mg/kg b.w. respectively. In the second phase of study, group C received the drug by PO at a dose of 5 mg/kg b.w. for 5 consecutive days. The eggs were collected for 30 days from the beginning of the experiment. Plasma, egg yolk and album samples were analysed using the HPLC-FL instrument. In the fourth study, 6 lactating goats were divided into 2 groups: group A (n = 3) and B (n = 3) where meloxicam was administered by IV and intramuscular (IM) routes at a dose of 0.5 mg/kg b.w. Milk samples were taken up to 168 h for quantification of drug residues in the organic matrix. The plasma and milk samples were analysed using the HPLC-DAD instrument